Assoc.Prof.Chanvit  Leelayuwat

PERSONAL
Name                                        Chanvit  LEELAYUWAT
Date of Birth                              15 March 1961
Place of Birth                            Bangkok, Thailand
Nationality                                 Thai
Marital Status                            Married with two children
Health                                       Excellent
Address                                                555/306 Saifon 2, Mitraphap Road, Amphur Muang Khon Kaen 40000 THAILAND.
Work   : 66(0)43-202089, 236905, 347482          Home:66(0)43-342216
FAX     : 66-(0)-43-202089, 347482, 236905
Mobile : 66(0)-1392 3802                           E-mail : chanvit@kku.ac.th

EDUCATIONAL BACKGROUND

TERTIARY EDUCATION
1979-1983                                 Mahidol University, Thailand : Bachelor of Science (Medical Technology) with First Class Honours
1983-1986                                 Mahidol University, Thailand : Master of Science (Clinical Pathology)
Thesis - Evaluation of antibody elution techniques using enzyme-linked antiglobulin test.
1990-1995                                 The University of Western Australia, Australia : Doctor of Philosophy (Molecular Immunology)
Thesis - Comparative Genomic Analysis of the central major histocompatibility complex.

ACADEMIC AWARDS                Golden Medal Prize for the best graduate in B.Sc. (Medical Technology) of the Mahidol University in 1983.
Excellence in Research Award 2005, Khon Kaen University

ROYAL MEDALS/AWARDS

2007                                         Knight Grand Cross (First Class) of the Most Noble Order of the Crown of Thailand.
2004                                         Knight Commander (Second Class) of the Most Exalted Order of the White Elephant.
2000                                         Knight Commander (Second Class) of the Most Noble Order of the Crown of Thailand.
1994                                         Commander (Third Class) of the Most Exalted Order of the White Elephant.
1992                                         Commander (Third Class) of the Most Noble Order of the Crown of Thailand.

CURRENT APPOINTMENT        Associate Professor (P.C.) 9, Project Director, The Centre for Research and Development of Medical Diagnostic Laboratories (CMDL), Associate Dean of Research, Graduate study and International Relation, Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen, Thailand.

PREVIOUS ACADEMIC APPOINTMENTS

1987                                         Lecturer grade (P.C.) 4, Department of Clinical Immunology, Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen, Thailand.

1989                                         Lecturer grade (P.C.) 5, Department of Clinical Immunology, Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen, Thailand.

1994                                         Ada Batholomew Research Fellow, The Centre for Cell and Molecular Biology and The Centre for Molecular Immunology and Instrumentation, The University of Western Australia, Perth, Western Australia.

1995                                         NHMRC Research Officer, The Centre for Molecular Immunology and Instrumentation, The University of Western Australia, Perth, Western Australia

1996                                         Lecturer grade (P.C.) 6, Department of Clinical Immunology, Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen, Thailand.

1997                                         Associate Dean of Research and Assistant Professor (P.C.) 6, Department of Clinical Immunology, Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen, Thailand.

1999                                         Associate Dean of Research, Assistant Professor (P.C.) 7, Department of Clinical Immunology, Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen, Thailand.

2000                                         Acting Deputy Head of Department of Clinical Immunology, Assistant Professor (P.C.) 7, Department of Clinical Immunology, Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen, Thailand.

2002                                         Deputy Head of Department of Clinical Immunology, Assistant Professor (P.C.) 8, Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen, Thailand.

2003                                         Project Director, The Centre for Research and Development of Medical Diagnostic Laboratories (CMDL), Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen, Thailand.

2006                                         Associate Professor (P.C. 9) , Project Director, The Centre for Research and Development of Medical Diagnostic Laboratories (CMDL), Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen, Thailand.

2008                                         Associate Dean of Research, Graduate study and International Relation, Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen, Thailand.

 

MEMBERSHIP OF PROFESSIONAL BODIES

The Council of the Medical Technologist Association of Thailand - Member
Australasian and South East Asian Tissue Typing Association (ASEATTA) - Councilor, Thailand (1999-2002)
The Allergy, Asthma and Immunology Society of Thailand – Academic subcommittee (2000-2002)

JOURNAL EDITORIAL BOARD
International
International Journal of Biomedical Sciences
Asian Pacific Journal of Allergy and Immunology
National
The Journal of Hematology and Transfusion Medicine
Journal of Medical Technology and Physical Therapy

INTERNATIONAL COMMITTEES

The 5th Asia and Oceania Histocompatibility Workshop and Conference (Secretary-General, Chairman of the Scientific Committee and Coordinator of WSVIII), Thailand.

The Australian National Health and Medical Research Council (NH&MRC) Independent Panel of Assessors (from 1995)

Chairman of the 2nd Congress of the Federation of Immunology Societies of Asia-Oceania (FIMSA) Satellite Workshop on “ MHC Typing and Analysis”  Khon Kaen University, Thailand, 28-30 January 2000

International Advisory Committee, The 7th Asia and Oceania Histocompatibility Workshop and Conference, Japan.

Scientific Advisory Committee, 14th International HLA and Immunogenetics Workshop 2005, Australia

Academic Subcommittee, the 5th World Melioidosis Congress 2007, 21-23 November 2007, Khon Kaen, Thailand

MAJOR ACADEMIC AND RESEARCH INTERESTS

Molecular biology and genetics of the immune system in infectious diseases, cancer and transplantation especially molecular interactions of NKG2D and NKG2D ligands relevant to diseases and transplantation

SCHOLARSHIPS AWARDED

1983-1986                     Mahidol Post-graduate Scholarship
1990-1994                     Equity and Merit Scholarship Scheme (EMSS; AIDAB)
1991                             Immunogenetics Research Foundation travel grant
1991                             Post-graduate student travel award (The University of Western Australia)
1994                             Immunogenetics Research Foundation travel grant
1994-1995                     Ada Batholomew Fellowship (The University of Western Australia)
1995                             Cold Spring Harbor Scholarship to attend the CSH Fall Course

  • KKU Travel Award for paper presentation (Khon Kaen University)
  • KKU Travel Award for paper presentation (Khon Kaen University)

1999-2002                     Thailand Research Fund Scholar

 

PROJECT GRANTS AWARDED

PERB1 (FGFR6) as a mechanism for MHC associations with non-immunological diseases associated with HLA-C or B (Dr PN Hollingsworth, Dr C Leelayuwat, Professor RL Dawkins : NH&MRC Grant No 941158, 1994-1996)

Characterisation of the PERB11 gene family in the MHC : Gene organisation and conservation towards function (Dr C Leelayuwat, Dr CS Witt, Associate Professor FT Christiansen and Professor RL Dawkins : NH&MRC Grant No 960578, 1996-1998)

Risk Factors in Melioidosis : Susceptible MHC haplotypes. (Principal Investigator: Thailand Research Fund, 1997-1998)

RAPD analysis of Burkhoderia pseudomallei (Principal Investigator: Thailand Research Fund, 1997-1998)

Evaluation of MHC Block Matching for Bone Marrow transplantation in Thailand. (Principal Investigator: National Research Council of Thailand, 1997-1998)

The Establishment of Regional Immunopathology and Immunogenetics Network (Co-investigator : AIDAB (AAECP) and KKU, 1997-1998)

Polymorphism of PERB11 in the Northeastern Thais. (Principal Investigator: Khon Kaen University Grant, National Research Council of Thailand, 1998)

The association of PERB11 polymorphism with infection and clinical manifestations in Melioidosis. (Principal Investigator: Khon Kaen University Grant, National Research Council of Thailand, 1999)

Characterisation of a novel gene family (PERB11/MIC) in the major histocompatibility complex : antibody production and identification of ligand peptide sequences. (TRF Research Scholar : Thailand Research Fund, 1999 - 2002)

Functional polymorphism of PERB11.1 (MICA), a ligand for activating NK cell receptor in development of cholangiocarcinoma. (Principal Investigator: Khon Kaen University Grant, National Research Council of Thailand, 2002-2003)

Polymorphisms of NKG2D and its ligands contributing to severity of dengue infection (Part I): Expression of NKG2D ligands on dengue infected cells. T2-program (WHO-NSTDA-TRF). 2004-2005

Soluble NKG2D ligands in cervical cancer (Principal Investigator: Khon Kaen University Grant, National Research Council of Thailand, 2005-2006)

Production and evaluation of molecular HLA typing kit for matching of tissue and organ transplantation
(Principal Investigator: National Research Council of Thailand, 2006-2007)

Expression of NKG2D ligands in cervical cancer (Principal Investigator: Khon Kaen University Grant, National Research Council of Thailand, 2007-2008)

Applications of Magnetic Nanoparticle in Medicine (National Research Council of Thailand, 2007-2008)

INTERNATIONAL CONFERENCES AND MINI SABBATICALS

1991     The ASEATTA Annual Scientific Meeting, Nakorn pathom, Thailand.
The 11th International Histocompatibility Workshop and Conference, Yokohama, Japan.
1992     The Lorne Genome Conference, Melbourne, Victoria, Australia.
Sabbatical (4 weeks) at WEHI and the Ludwig Institute for Cancer Research, Melbourne, Australia.
1993     The 4th Asia Oceania Histocompatibility Workshop and Conference, Perth, Western Australia,
1994     The 13th International Immunology and Diabetes Workshop, Montvillargene, France.
Sabbatical (4 weeks) at the Basel Institute for Immunology, Basel, Switzerland.
1995     Immunex Research and Development Corporation, Seattle, WA, USA, 1-7 November.
Cold Spring Harbor Laboratory : The Phage Display of Combinatorial Antibody Libraries Course, New York,  USA, 8-21 November.
The 5th Asia Oceania Histocompatibility Workshop and Conference, Bangkok, Thailand, 10-13December.  
1996     12th International Histocompatibility Workshop and Conference, Paris, France, 10-13 June.
1997     The Centre for Molecular Immunology and Instrumentation, The University of Western Australia, Australia, 23 June - 29 July
1998     The Centre for Molecular Immunology and Instrumentation, The University of Western Australia, Australia, 9 April – 20 May
1998     6th Asia-Oceania Histocompatibility Workshop and 1998 Annual Scientific Meeting of Australsian and South East Asian Tissue Typing Association (ASEATTA), New Delhi, India, 29 October - 1 November 1998
10th International Congress of Immunology, New Delhi, India, 1-6 November 1998
1999     The AAECP and CMII/IRF Workshop on antibody production, The Centre for Molecular Immunology, The University of Western Australia, Australia,  8-12 April 1999
The CMII/IRF Scientific Weekend Retreat, The Centre for Molecular Immunology, The University of Western Australia, Australia, 9-11 July 1999

  • The 2nd Congress of the Federation of Immunology Societies of Asia-Oceania (FIMSA), Imperial Queen’s Park Hotel, Bangkok, Thailand, 23-27 January 2000

The Annual Scientific Meeting of ASEATTA 2000, Perth, Australia, 1-3 December 2000
2001     The ASEATTA Annual Scientific Meeting and Chromosome 6 Workshop, Courtyard Marriott Hotel, Suffer paradise, Gold coast, Queensland, Australia, 19-24 November 2001
2002     Division of Immunology, Department of Pathology, University of Cambridge, UK , 6-25 May 2002
The 27th Congress of the International Society of Blood Transfusion, Vancouver Exhibition Center, Vancouver, Canada, 23-29 August 2002
The 26th Annual Scientific Meeting of Australsian and South East Asian Tissue Typing Association (ASEATTA), Adelaide, Australia, November 22-24, 2002
2003     The Roswell Park Cancer Institute, Buffalo, New York, USA. 7-13 January, 2003.
The Type 1 Diabetes Genetic Consortium Meeting, Bethesda, Maryland, Washinton, USA. 13 January, 2003.
The 15th European Federation of Immunology Societies Meeting. Rhodes, Greece, 7-12 June, 2003.
Asian Pacific Network of Type 1 Diabetes Genetic Consortium, Cairns, Australia, 19-20 June, 2003.
Academic Visit at The Kumamoto University, Kyushu Tokai University, Japan, 18-22 August, 2003.
33rd Annual Scientific Meeting of the Australasian Society for Immunology, Perth, Western Australia, 7-11 December 2003
2004     12th International Congress of Immunology and 4th Annual Conference of FOCIS, July 18-23, 2004 - MONTR?AL – CANADA
            Academic Visit and collaborative research at Cornell University, Ithaca, USA  and Pasteur Institute, Paris, France, 5-17 September, 2004
2005    Academic Visit and collaborative research at The Kumamoto University, Japan, 18-28 June, 2005
34rd Annual Scientific Meeting of the Australasian Society for Immunology and 14th International HLA and Immunogenetics Workshop and Conference, Australia, 29 November - 7 December 2005
2006     Academic Visit and collaborative research at The Kumamoto University, Japan, 19-24 March, 2006
Academic Visit and collaborative research at The Roswell Park Cancer Institute, Buffalo, New York, USA.  22-29 April, 2006
Academic Visit and collaborative research at Institut f?r Biochemie, Westf?lische Wilhelms-Universit?t M?nster, M?nster, Germany.  2-7 May, 2006
European Federal of Immunogenetics (EFI) : 20th  Inmunogenetics and Histocompatibility Conference, Oslo, Norway, 7-11 June 2006.
2007     Academic visit at the Institut Pastuer, Paris, France, April 23 – May 9, 2007
The 7th Annual Meeting of the Federation of Clinical Immunology Societies (FOCIS) 2007, June 7-11, 2007, San Diego, USA
2008     Academic Visit and collaborative research at The Kumamoto University, Japan, 10-15 March, 2008
2009     Kumamoto University, Kumamoto Health Science University, Keio University, Fujita Health University, National Institute of Infectious Diseases, Japan, 18-24 January, 2009.

INVITED SPEAKER

1995     New genes from DNA analysis at "Presentation of Science" Annual General Meeting of the Immunogenetics Research Foundation, Perth, Western Australia, 26 June.

            New non-HLA MHC genes and organisation at "The Fifth Asia and Oceania Histocompatibility Complex Workshop and Conference" Bangkok, Thailand, 10-13 December.

1996     New genes relevant to HIV and AIDS at "Presentation of Science" Annual General Meeting of the Immunogenetics Research Foundation, Perth, Western Australia.

1997     “Recent Concepts in the Major Histocompatibility Complex” at the Faculty of Medicine, Chiangmai University (29 August ) and the Thai Red Cross Society (19 September), Thailand

1998     “Structure and Function of Non-classical Class I - like MHC genes : Relevance to disease.” In the Interactive Workshop “Non-classical MHC genes and Proteins” at the 10th International Congress of Immunology, 1-7 November 1998, New Delhi, India

1999     “A novel gene family (PERB11/MIC) in the major histocompatibility complex” at The Annual General Meeting of The Allergy and Immunology Society of Thailand, Siam City Hotel, Thailand, 29 October 1999

  • “Polymorphisms, Evolution and functions of the PERB11/MIC gene family in the MHC : Relevance to diseases.” at The 2nd Congress of the Federation of Immunology Societies of Asia-Oceania (FIMSA), Imperial Queen’s Park Hotel, Bangkok, Thailand, 23-27 January 2000

 

  • “Molecular Interaction of an activating receptor (NKG2D) and its ligands : Relevant to deseases.” at The Annual General Meeting of The Allergy and Immunology Society of Thailand, Queen Sirikit National Convention Center, Bangkok, Thailand, 7-8 November, 2002.

2003     “Molecular Techniques in Routine Laboratories : Molecular HLA Typing” at the Annual Meeting of Medical Technologist Association of Thailand, Ambassador City, Pattaya, Thailand, 29 April -1 May 2003.

2004     “Major histocompatibility (MHC) typing and analysis in bone marrow transplantation : Relevance of matching of MHC blocks and killer immunoglobulin-like receptor (KIR) and ligands” at the 10th ASEAN Conference in Medical Laboratory Technology, Lotus Hotel Pang Suan Kaew, Chiang Mai, Thailand, April 26-30, 2004.

2009     “Role of Human Leukocyte Antigen (HLA) in Immune Responses: Relevance to Immune Mediated Adverse Drug Effects (IMADE)” at the 31st Pharmacological and Therapeutic Society of Thailand Meeting, Khon Kaen, Thialand, 18-20 March 2009.

2009     “NKG2D and Cancer” at the 25th Congress on Allergy and Immunology, Sofitel Centara Grand Bangkok, Thailand, 30 April –May 1, 2009.

PUBLICATIONS
National
1.             Romphruk, A., Urwijitaroon, Y., Leelayuwat, C and Meesang, M. Modified Microplate Technique for Rh(D) Typing. J Med Tech Ass Thailand  20:123-127, 1992
2.             Cheepsattayakorn R, L Fongsatitkul , AV. Romphruk, T Bhoopat, HF. Steger, C Leelayuwat and S Chomsook. The B weak Phenotype in a Thai Family. Thai Journal of Hematology and Transfusion Medicine  12: 77-90, 2002
3.             Leelayuwat C and Romphruk AV. Molecular techniques in routine medical laboratory : HLA typing by KKU SSP typing kit. J Med Tech Assoc Thailand 31: 292-307, 2003.
4.             Wanrum S, Limpaiboon T, Leelayuwat C, Yaenyao P, Luanratanakorn S and Jearanaikoon P. Development of method for integrated HPV 16 detection in cervical carcinoma using multiplex quantitative PCR. J Med Tech Phys Ther  19: 6-15, 2007.
5.             Yussara M, Leelayuwat C, Urwijitaroon Y, Barusrux S, Promwong J, Tadein R. Prevalence of hepatitis virus infections in the first donating blood donors at the regional Yala Hospital during 2001-2005. J Med Tech Phys Ther  19: 16-24, 2007.
6.             Jearanaikoon P, Nattasirikul N, Kaewpo W, Wanrum S, Leelayuwat C, Puapairoj A, Limpaiboon T. Tapasin expression in cervical cancinoma. J Med Tech Phys Ther  19: 55-63, 2007.
7.             Barusrux S, Wongsena W, Leelayuwat C, Puapairoj A, Jearanaigoon P, Yuenyao P, Romphruk A, Nanok C. Immunohistochemical staining for MICA expression in cervical cancer cells. J Health Res 22: 35-42, 2008.
8.             Wilai Pattoom, Yupa Urwijitaroon, Pranom Pattoom, Sasithon Padsan, Chanvit Leelayuwat  and Kunnika Kuaha.  Prevalence of HIV, HBV and HCV Infections in First-time Blood Donors at Somdejphrachaotaksin Maharaj Hospital, Tak Province, During 2001-2005.. Thai Journal of Hematology and Transfusion Medicine  18 ( 2), 101-108, 2008
9.             Duangchanchot M, Romphruk A, Puapairoj C, Kongmaroeng C, Leelayuwat C and Romphruk AV. Detection of HLA-B*27 in Thais, Burmese and Karens. J Med Tech Phys Ther  20: 194-200, 2008.

 

International
1.             Lertmemongkolchai, G., Manmontri, W., Leelayuwat, C., Romphruk, A. and Waropastrakul, S. Immunoblot analysis to demonstrate antigenic variability of clinical isolated Pseudomonas pseudomallei.  Asian Pac J Allergy Immunol 9:5-8, 1991.

2.             Leelayuwat, C., Abraham, L.J., Tabarias, H., Christiansen, F.T. and Dawkins, R.L.  Genomic organisation of a polymorphic duplicated region centromeric of HLA?B. Immunogenetics 36: 208?212, 1992.

3.             Leelayuwat, C., Abraham, L.J., Tabarias, H.A., Mann, A.J., Grimsley, G., Degli-Esposti, M.A., Zhang, W.J., Christiansen, F.T. and Dawkins, R.L. Haplospecific polymorphism in a duplicated region centromeric of HLA?B. In K. Tsuji, M. Aizawa and T. Sasazuki  (eds.) HLA 1991, Vol 2, pp. 172?175,Oxford University Press, New York, 1992.

4.             Abraham, L.J., Leelayuwat, C., Grimsley, G., Degli?Esposti, M.A., Mann, A., Zhang, W.J., Christiansen, F.T. and Dawkins, R.L.  Sequence differences between HLA?B and TNF distinguish different MHC ancestral haplotypes. Tissue Antigens 39: 117?121, 1992.

5.             Degli?Esposti, M.A., Leelayuwat, C. and Dawkins, R.L.  Ancestral haplotypes carry haplotypic and haplospecific polymorphisms of BAT1: Possible relevance to autoimmune disease. Eur J Immunogenet 19: 121?127, 1992.

6.             Zhang, W.J., Wu, X., Witt, C., Leelayuwat, C., Abraham, L.J., Grimsley, G., Degli-Esposti, M.A., Christiansen, F.T. and Dawkins, R.L. New genomic markers between the HLA?B and TNF loci define MHC ancestral haplotypes. In K. Tsuji, M. Aizawa and T. Sasazuki  (eds.) HLA 1991, Vol 2, Oxford University Press, New York, 1992, p 176?179. 

7.             Leelayuwat, C., Zhang, W.J., Abraham, L.J., Townend, D.C., Gaudieri, G. and Dawkins, R.L.  Differences in the central major histocompatibility complex between humans and chimpanzees: Implications for development of autoimmunity and acquired immune deficiency syndrome. Hum Immunol   38:30-41, 1993.

8.             Marshall, B., Leelayuwat, C., Abraham, L.J., Pinelli, M. and Dawkins, R.L.  Large transcripts and sequence from a polymorphic 170 kb MHC region implicated in susceptibility to autoimmune disease. Immunogenetics 39:15-20, 1993.

9.             Marshall, B., Leelayuwat, C., Degli?Esposti, M.A., Pinelli, M., Abraham, L.J. and Dawkins, R.L. New Major Histocompatibility Complex Genes. Hum Immunol   38:25-29, 1993.

10.          Abraham, L.J., Grimsley, G., Leelayuwat, C., Townend, D.C., Pinelli, M., Christiansen, F.T. and Dawkins, R.L.  A Region Centromeric of the Major Histocompatibility Complex Class I Region Is as Highly Polymorphic as HLA-B: Implications for Recombination. Hum Immunol   38:75-82, 1993.

11.          Leelayuwat, C., Degli?Esposti, M.A.,Taylor, E., Abraham, L.J. and Dawkins, R.L.  PCR SSCP reveals haplotype related polymorphism of PERB1 : A new marker for MHC ? block typing. Eur J Immunogenet 21:431-446, 1994.

12.          Leelayuwat, C., Townend, D.C., Degli?Esposti, M.A, Abraham, L.J. and Dawkins, R.L.  A new polymorphic and multicopy MHC gene family related to nonmammalian class I. Immunogenetics 40:339-351, 1994.

13.          Degli-Esposti, M.A, Leelayuwat, C., Daly, L., Carcassi, C., Contu, L., Versluis, L., Tilanus, M.G.J., Dawkins, R.L. UPDATED CHARACTERIZATION OF ANCESTRAL HAPLOTYPES using the Fourth Asia-Oceania Histocompatibility Workshop Panel. Human Immunol  44: 12-18, 1995.

14           Leelayuwat, C., Pinelli, M. and Dawkins, R.L. Clustering of  diverse replicated sequences in the major histocompatibility complex (MHC) : Evidence for en bloc duplication. J Immunol 155: 692-698, 1995.

15.          Leelayuwat, C.,  Abraham, L.J., Pinelli, M., Townend, D.C., Wilks, A.F. and Dawkins, R.L.   The primate MHC contains sequences related to the fibroblast growth factor receptor gene family. Tissue Antigens  48: 59-64, 1996.

16.          Leelayuwat, C., Hollinsworth, P., Pummer, S., Lertmemongkolchai, G., Graeme, T., Mullberg, J., Witt, C., Kaufman, J., Cosman, D., and Dawkins, R.L. Antibody reactivity profiles following immunisation with diverse peptides of the PERB11 (MIC) family. Clin Exp Immunol 106:568-576, 1996.

17.          Townend, D., Gaudieri, S., Leelayuwat, C., Tay, G., Degli-Esposti, M. and Dawkins, R.L.  Genomic strucutre of a 200kb region between HLA-C and TNF:  Implications in Transplantation, Autoimmune diseases and Evolution.  DNA Sequence 7 : 55-59, 1996.

18.          Romphruk,  A., Barusrux, S., Puapairoj, C., Urwijitaroon, Y., Romphruk, Ar. and Leelayuwat, C. Distribution of HLA-A and B in Northeastern Thais. J Med Assoc Thai  79: 732-736,1996.

19           Leelayuwat C, Srisuk T, Paechaiyaphum R, Limpaiboon T, Romphruk Ar and Romphruk Am. Production and Evaluation of Taq DNA Polymerase.  J Med Assoc Thai 80 : Suppl. 1. S129-S137, 1997.

20.          Leelayuwat, C., Gaudieri, S., Mullberg, J., Cosman, D., and Dawkins, R.L.  Interlocus sequence analysis of the PERB11 gene family : Implication for rapid diversification to different functions. In D Charron ed. Proceedings of the 12th IHWC Genetic Diversity of HLA: Functional and Medical Implications. Vol II, Medical and Scientific International Publisher, Paris, 1997, p217-219.

21.          Gaudieri, S., Leelayuwat, C., Townend, D., Mullberg, J., Cosman, D. and Dawkins, R.L. Allelic and interlocus comparison of the PERB11 multigene family in the MHC. Immunogenetics 45 : 209-216, 1997.

22.          Tay, G.K., Leelayuwat, C., Chorney M.J., Cattley S.K., Hollingsworth, P.N., Witt, C.S., Daly,  L.N., Hughes, A. and Dawkins R.L. The MHC contains multiple genes potentially relevant to hemochromatosis. Immunogenetics 45 : 336-340, 1997.

23.          Gaudieri, S, Leelayuwat, C, Townend, D,  Kulski, JK, and Dawkins, RL. Genomic characterisation of the region between HLA-B and TNF: Implications for the evolution of multicopy gene families. J Mol Evol  44 : 147-154, 1997.

24.          Gaudieri, S., Leelayuwat, C., Tay, G., Townend, D. and Dawkins, R.L.  The MHC contains polymorphic genomic sequences which are shuffled by recombination to form ethnic specific haplotypes.  Mol Biol  Evol  45 : 17-23, 1997.

25.     Romphruk AV, Leelayuwat C, Barusrux S, Puapairoj C, Romphruk A and Urwijitaroon Y. DNA Typing of the HLA-A, -B and -C Genes : Possible MHC Class I Haplotypes in the Northeastern-Thais.  J Med Assoc Thai  80 : Suppl. 1. S13-S19, 1997.

  •  

26           Dawkins R, Leelayuwat C, Gaudieri S, Tay G, Hui J, Cattley S, Martinez P and Kulski J. Genomics of the major histocompatibility complex: haplotypes, duplication, retroviruses and disease. Immunological Reviews  167, pp.275-304, 1999

  • Allcock RJ, Price P, Gaudieri S, Leelayuwat C, Witt CS, Dawkins RL. Characterisation of the Human Central MHC Gene, BAT1: Genomic Structure and Expression. Exp Clin Immunogenet 16(2):98-106, 1999

 

  • Romphruk AV, Puapairoj C, Romphruk A,  Barusrux S, Urwijitaroon Y and Leelayuwat C. The distributions of HLA-DRB1/DQB1 alleles and haplotypes in the Northeastern Thai population : Indicative of a distinct Thai population with Chinese admixtures in the Central Thais. Eur J Immunogenet  26(2) : 129-134, 1999
  • Cattley SK, Longman N, Gaudieri S, Dawkins RL and Leelayuwat C. Phylogenetic analysis of primate PERB11(MIC) sequences suggests that the representation of the gene family differs in different primates. Eur J Immunogenet  26(2) : 233-238, 1999
  •  
  • Tay GK, Hui J, Gaudieri S, Schmitt-Egenolf, Martinez OP,  Leelayuwat C, Williamson JF, Eiermann TH and Dawkins RL. PERB11 (MIC) : a polymorphic MHC gene is expressed in skin and single nucleotide polymorphisms are associated with psoriasis. Clin Exp Immunol 119 : 553-558, 2000.

 

  • Dunn DS, Williamson JF, Cattley SK, Tay GK, Guadieri S, Leelayuwat C and Dawkins RL. Co-evolution of HLA-B and PERB11.1 (MICA) : Significance of independent triplet expansion within the transmembrane region of PERB11.1 (MICA). J Mol Evol  50 :359-65., 2000.
  • Leelayuwat C, Romphruk A, Lulitanond A, Trakulsomboon S and Dramalikitkul V.  Genotype analysis of Burkolderia pseudomallei using randomly amplified polymorphic DNA (RAPD). Acta Tropica 77 : 229-237,  2000.

 

  • Romphruk AV, Akahat J, Srivanichrak P, Puapairoj C, Romphruk A and Leelayuwat C. Genotyping of human platelet antigens in ethnic northeastern Thais by the polymerase chain reaction – sequence specific primer technique.  J Med Assoc Thai  83 : 1333-1339, 2000.
  • Komatsu-Wakui M, Tokunaga K, Ishikawa Y, Leelayuwat C, Kashiwase K, Tanaka H, Moriyama S, Nakajima F, Park MH, Jia GJ, Chimge NO, Sideltseva EW and Juji T. Wide distribution of the MICA-MICB null haplotype in East Asians. Tissue Antigens 57: 1-8, 2001.

 

  • A.V. Romphruk, T.K. Naruse, A. Romphruk, H. Kawata, C. Puapairoj, J.K. Kulski, C. Leelayuwat, H.Inoko. Diversity of MICA (PERB11.1) and HLA haplotypes in Northeastern Thais. Tissue Antigens 58: 83-89, 2001.
  • J K Kulski, D Dunn, J Hui, P Martinez, AV Romphruk, C Leelayuwat, G Tay,  A Oka, H Inoko. Alu polymorphism within the MICB gene and association with MHC haplotypes. Immunogenetics 53: 975-979, 2002.

 

  • C Choonhakarn, AV Romphruk, C Puapairoj, K Jirarattanapochai, A Romphruk and C Leelayuwat. Haplotype associations of the major histocompatibility complex with psoriasis in northeastern-Thais. Int J Dermatol 41:330-334, 2002.
  • A.V. Romphruk, A. Oka, A. Romphruk, M. Tomizawa, C. Choonhakarn, T.K.  Naruse, C. Puapairoj, G Tamiya, C. Leelayuwat, H. Inoko Corneodesmosin gene: no evidence supported for PSORS 1 gene in Northeastern Thai psoriasis patients. Tissue Antigens 62;217-224, 2003.

 

  • A. Romphruk, K. Phonaegn, J. Chotechai, C. Puapairoj, C. Leelayuwat,  A.V. Romphruk. HLA-B*15 subtypes in Northeastern-Thais. Eur J Immunogenet 30:153-158, 2003.
  • A.V. Romphruk, A. Romphruk, C. Choonhakarn, C. Puapairoj, H. Inoko and C. Leelayuwat. MHC Class I Chain-Related Gene A (MICA) In Thai Psoriasis Patients:  MICA Association as a Part of HLA-B-Cw Haplotypes. Tissue Antigens 63: 547-54, 2004

 

  • D.S. Dunn, A.V. Romphruk, C. Leelayuwat, M. Bellgard and J.K. Kulski. Polymorphic Alu insertions and their associations with MHC class I alleles and haplotypes in the Northeastern Thais. Annals Hum Genetics 69: 364-372, 2005.
  • Kitcharoen, K, Witt, CS, Romphruk, AV, Christiansen, FT and Leelayuwat, C. MICA, MICB and MHC beta block matching in bone marrow transplantation: Relevance to transplantation outcome. Human Immunol 67:238-246, 2006.

 

  • Supaprom C,  Wang D, Leelayuwat C, Thaewpia W, Susaengrat W, Koh V, Ooi EE,  Lertmenongkolchai G and Liu Y. Development of Real-Time PCR Assays and Evaluation of Their Potential Use fpr Rapid Detection of Buukholderia pseudomallei in Clinical Blood Specimens. J Clin Microbiol 45; 2894-2901, 2007

 

  • Tongchusak S, Leelayuwat C, Brusic V, Chaiyaroj SC.  In silico prediction and immunological validation of common HLA-DRB1-restricted T cell epitopes of Candida albicans secretory aspartyl proteinase 2.  Microbiol Immunol 52(4):231-242, 2008.          
  • Jumnainsong A, Jearanaikoon P, Khahmahpahte S, Wongsena W, Romphruk AV, Chumworathayi B, Vaeteewoottacharn K, Ponglikitmongkol M, Romphruk A, Leelayuwat C. Associations of MICB with cervical cancer in northeastern Thais: Identification of MICB motifs influencing NK cell activation. Clin Exp Immunol 153: 205-213, 2008

 

  • Wongsena W, Sconocchia G, Cho HS, Chang CC, Wang X, Klumkrathok K, Ferrone S, and Leelayuwat C. Production and characterization of monoclonal antibodies against MHC class I chain related gene A (MICA). Tissue Antigens 72(5):431-40, 2008.
  • Duangchanchot M, Puapairoj C, Romphruk A, Kongmaroeng C, Leelayuwat C , Romphruk AV. HLA-B*27 subtypes in northern and northeastern Thais, Karens  and Bamars determined by a high resolution PCR-SSP technique. Tissue Antigens 73: 590-594, 2009.

 

  •                 Kongmaroeng C, Romphruk  A, Ruangwerayut  R, Paupairoj C, Leelayuwat C, Inoko H, Romphruk AV. HLA-B*15 subtypes in Burmese population by SBT. Tissue Antigens 74; 164-167, 2009
  • Chan-on W, Kuwahara K, Kobayashi N, Ohta K, Shimazaki T, Sripa B, Leelayuwat C and Sakaguchi N. Cholangiocarcinomas associated with long-term inflammation express the activation-induced cytidine deaminase and germinal center-associated nuclear protein involved in immunoglobulin V-region diversification. Int J Oncol 35: 287-295, 2009

 

  • Wanram S, Limpaiboon T, Leelayuwat C, Yuenyao P, Guiney DG, Lulitanond V and Jearanaikoon P . Prognostic significance of human papillomavirus type 16 physical status and viral load in predicting clinical outcome of cervical cancer. Am J Obstret Gynnecol 2009 Jul;201(1):79.e1-7. Epub 2009.
  • Romphruk AV, Romphruk A, Naruse TK, Raroengjai S, Puapairoj C, Inoko H and Leelayuwat C. Polymorphisms of NKG2D ligands: diverse RAET1/ULBP genes in northeastern Thais. Immunogenetics 61: 611-617, 2009; DOI: 10.1007/s00251-009-0394-7

  • REFEREES

 

Academic and Professional

1.             Professor Roger L Dawkins
The Centre for Molecular Immunology and Instrumentation
The University of Western Australia
GPO Box 507, SUBIACO, WA 6008 AUSTRALIA 
Telephone            61-8-934 64414                   Facsimile               61-8-934 64578
E-mail    dawkins@erythema.cmii.uwa.edu.au

2.             Dr. Andrew F Wilks
The Ludwig Institute for Cancer Research
Melbourne Tumor Biology Branch
Royal Melbourne Hospital
                VICTORIA 3050  AUSTRALIA
Telephone            61-03-3471938                    Facsimile               61-03-3471938

3.             Professor Pimol Chiewsilp
National Blood Centre
Thai Red Cross Society
Henri Dunant Road
                BANGKOK  10330  THAILAND
Telephone            66-2-251 3111 ex 191        Facsimile               66-2-255 5558
E-mail                    rapcw@mucc.mahidol.ac.th

4.             Dr Jim Kaufman
Institute for Animal Health
Campton RG 20 7NN
                BERKSHIRE, UNITED KINGDOM
Telephone            44-1365-577234                 Facsimile               44-1365-577263

Faculty of Associated Medical Sciences Khon kaen University Tel : 0 4320 2399, 0 4334 7482 International Phone/Fax: +66 43 202399, +66 43 347482